Hi everyone!
I have imported reference sequences and taxonomy and now want to filter out sequences that are not Glomeromycota. When I use feature-classifier classify-consensus-blast
qiime feature-classifier classify-consensus-blast
--i-query frequency-filtered-rep-seqs.qza
--i-reference-reads cacao_seqs.qza
--i-reference-taxonomy taxonomy.qza
--p-query-cov 0.95
--p-perc-identity 0.95 \
--p-maxaccepts 1 \
--o-classification Blast_rep_taxonomy-CQ-95-95-1.qza
I always get this error:
(1/3) Missing option '--o-classification'. ("--output-dir" may also be used)
(2/3) Missing option '--o-search-results'. ("--output-dir" may also be used)
(3/3) Got unexpected extra argument ( )
frequency-filtered-rep-seqs.qza (82.5 KB)
cacao_seqs.qza (1.2 MB)
taxonomy.qza (10.0 KB)
What am I doing wrong?
Does anyone know what the reason could be?
I am grateful for any advice.
kind regards
Franzi