feature-classifier extract-reads v3 v4

General Discussion
2

Hi @Linda_Abenaim :slightly_smiling_face:

There is a lot of advice on the forum about training databases (see here and here). The Qiime2 tutorial documents also have some useful information in the notes sections to get you thinking about your own sequences and how to make an informed decision.

Regarding what inputs are mandatory or not, you can look at the documentation and it will tell you which parameters are required or not. For example the feature-classifier extract-reads document describes the inputs (I've only pasted the top part here):

Inputs:
  --i-sequences ARTIFACT FeatureData[Sequence]   [required]
Parameters:
  --p-f-primer TEXT   forward primer sequence (5' -> 3').       [required]
  --p-r-primer TEXT   reverse primer sequence (5' -> 3'). 
                      Do not use reverse-complemented primer sequence.  [required]

These inputs are required, meaning they are mandatory and if you scroll further down on that document page, you'll see the output is also required as well (makes sense, you need to output the data :laughing:) .

So have a deeper look about and if you are still stuck after looking through these, post again and someone can help :+1:

best,

Vic

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