Feature Classifier: Extract Reads Help

[L_Zh (Lijun)]

my design cover V1 V2 regions, I want to generate reference data as below,

qiime feature-classifier extract-reads
–i-sequences 99__otus.qza
–p-f-primer AGAGTTTGATCMTGGCTCAG
–p-r-primer ACTGCTGCSYCCCGTAGGAGTCT
–p-trunc-len 308
–o-reads ref-seqs-V12.qza

The reference shouldn’t include forward (AGAGTTTGATCMTGGCTCAG) and revise primer (ACTGCTGCSYCCCGTAGGAGTCT), right? Here, I limited the length to 308, and want to exclude the v1, v2 designed primer.

Thanks for the help.

Best

LZ

[BenKaehler]

Hi @L_Zh, that looks fine to me. Was there a problem?