my design cover V1 V2 regions, I want to generate reference data as below,
qiime feature-classifier extract-reads
–i-sequences 99__otus.qza
–p-f-primer AGAGTTTGATCMTGGCTCAG
–p-r-primer ACTGCTGCSYCCCGTAGGAGTCT
–p-trunc-len 308
–o-reads ref-seqs-V12.qza
The reference shouldn’t include forward (AGAGTTTGATCMTGGCTCAG) and revise primer (ACTGCTGCSYCCCGTAGGAGTCT), right? Here, I limited the length to 308, and want to exclude the v1, v2 designed primer.
Thanks for the help.
Best
LZ