my design cover V1 V2 regions, I want to generate reference data as below,
qiime feature-classifier extract-reads
The reference shouldn’t include forward (AGAGTTTGATCMTGGCTCAG) and revise primer (ACTGCTGCSYCCCGTAGGAGTCT), right? Here, I limited the length to 308, and want to exclude the v1, v2 designed primer.
Thanks for the help.