my design cover V1 V2 regions, I want to generate reference data as below,
qiime feature-classifier extract-reads
--i-sequences 99__otus.qza
--p-f-primer AGAGTTTGATCMTGGCTCAG
--p-r-primer ACTGCTGCSYCCCGTAGGAGTCT
--p-trunc-len 308
--o-reads ref-seqs-V12.qza
The reference shouldn't include forward (AGAGTTTGATCMTGGCTCAG) and revise primer (ACTGCTGCSYCCCGTAGGAGTCT), right? Here, I limited the length to 308, and want to exclude the v1, v2 designed primer.
Thanks for the help.
Best
LZ