Hi all,
I am currently training the classifier and encountered a problem very similar to that of another user here. However, when I looked at my files and try to resolve the problem using their solution, I still cannot solve it. What I did was as follows:
Extract the reference reads from Greengenes reference database:
qiime feature-classifier extract-reads \
--i-sequences 2022.10.seqs.fna.qza \
--p-f-primer ACTCCTACGGGAGGCAGCAG \
--p-r-primer GGACTACHVGGGTWTCTAAT \
--p-trunc-len 446 \
--p-min-length 100 \
--p-max-length 450 \
--o-reads reference-seqs.qza
Training the classifier:
qiime feature-classifier fit-classifier-naive-bayes \
--i-reference-reads reference-seqs.qza \
--i-reference-taxonomy ref-tax.qza \
--o-classifier classifier.qza
--verbose
And the problem returned is:
Plugin error from feature-classifier:
not enough values to unpack (expected 2, got 0)
I tried to open the input files (i.e., the reference-seqs.qza
and the ref-tax.qza
) and see if the problem lies in the # character as in the post above, but I see none. Could you please assist me to solve this problem?
My files are a little bit too big, so I uploaded them here.
Thanks a lot for your assistance!!