Failure to plot Phylogenetic Tree

[Ayazaeroth (Saif M Arosh)]

Hello,
I was going through [Tutorial: Integrating QIIME2 and R for data visualization and analysis using qiime2R] (Tutorial: Integrating QIIME2 and R for data visualization and analysis using qiime2R) and stumbled upon a few errors. When running the codes for plotting the phylogenetic tree, it says-

Error in UseMethod("left_join") :
no applicable method for 'left_join' applied to an object of class "waiver"
This particular code for plotting the tree is very confusing to me. I'm attaching the files I used and the pictures of the error.
rooted-tree.qza (50.5 KB)
q2r_taxonomy.qza (70.0 KB)
q2r_table.qza (52.4 KB)
sample-metadata.tsv (2.0 KB)
differentials.qza (108.2 KB)

Changing the environment pannel to this-

Would be great help if anybody could point out what am I doing wrong?

And while running qiime2-ALDEx2 and using extract-differences function, I had to change the significance level to 0.55, it wouldn't work under that value. Why is that?

Pardon my naivety, but I just want to understand the codes.

[jbisanz (Jordan Bisanz)]

Not 100% sure, but it also looks like there are not any overlapping ASVs between your tree and your differentials.

[Ayazaeroth (Saif M Arosh)]

Thanks @jbisanz , looks like I mixed it up with another tree file. Sorry for your troubles