Facing Problem in taxonomic classifier

[Mehrbod_Estaki]

Hi @SAMRENDRA01,

I have already answered your original question in another thread very recently:

@Mehrbod_Estaki

I would recommend asking your sequencing facility or whoever did the amplicon preparation for this information. It will be required for publication anyways so you might as well get it. If for some strange reason this information is lost, you can always use a generic classifier that isn’t trained on your specific primers, albeit with slightly less accuracy. This is available on the data resource page .

You have also already asked for this classifer on another post, please avoid cross-posting questions:

@Francisco

@SAMRENDRA01

2. please provide me v3 v4 region silva classifier.

from here: SILVA 138 Classifiers
and i recommend this file (but its up to you!)
SILVA-138-SSURef-noSpeciesLabels-full-length-classifier.qza

And also, the link I provided you earlier to the data-resource page has a note on how to train your own classifier:

Note

Taxonomic classifiers perform best when they are trained based on your specific sample preparation and sequencing parameters, including the primers that were used for amplification and the length of your sequence reads. Therefore in general you should follow the instructions in Training feature classifiers with q2-feature-classifier to train your own taxonomic classifiers (for example, from the marker gene reference databases below).

Please follow the instructions starting there and please be more mindful of your approach in using the forum in the future.