Thanks for accepting me to qiime2forum.
i tried to add observation taxonomy metadata to the feature-table.biom , everything looking good but when i convert the biom file to tsv i cant see taxonomy added to the feature biom file…can you help please
i followed all the stages suggested by https://forum.qiime2.org/u/thermokarst
Exporting and modifying BIOM tables (e.g. adding taxonomy annotations)
This is an issue with biom-format, not with QIIME 2, so I am not too familiar with troubleshooting this if that does not work.
I hope that helps!
yes i added --header-key taxonomy its worked now.
But some sometimes instead putting taxonomy on the last column it puts taxonomy under the first column and named #OTU ID instead of taxonomy…very strange to me …
As far as I know, that should only happen if your feature table was “collapsed” (e.g., with
qiime feature-table collapse) based on taxonomic classifications, and biom should not do that during metadata annotation or conversion.
Thank you so much…I followed your suggestions …now its working correctly.