Hello all, I'm still a newbie,I'm trying to use qiime2 in a miniconda3 environment, and I tried to run dada2 following this script:
time qiime dada2 denoise-paired \
--i-demultiplexed-seqs demux.qza
--p-n-threads 16
--p-trim-left-f 29 --p-trim-left-r 20
--p-trunc-len-f 0 --p-trunc-len-r 0
--o-table dada2-table.qza
--o-representative-sequences dada2-rep-seqs.qza
--o-denoising-stats denoising-stats.qza
It said this afterwards:
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Debug info has been saved to /tmp/qiime2-q2cli-err-q70ufed2.log
And here's what's inside the debug file:
Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.
Command: run_dada_paired.R /tmp/tmpei0hpdw4/forward /tmp/tmpei0hpdw4/reverse /tmp/tmpei0hpdw4/output.tsv.biom /tmp/tmpei0hpdw4/track.tsv /tmp/tmpei0hpdw4/filt_f /tmp/tmpei0hpdw4/filt_r 0 0 29 20 2.0 2.0 2 independent consensus 1.0 16 1000000
R version 4.0.2 (2020-06-22)
Loading required package: Rcpp
DADA2: 1.18.0 / Rcpp: 1.0.6 / RcppParallel: 5.0.2
- Filtering
*** caught segfault ***
address 0x7f804647a000, cause 'invalid permissions'
*** caught segfault ***
address 0x7f8043044000, cause 'invalid permissions'
*** caught segfault ***
address 0x7f8048348000, cause 'invalid permissions'
*** caught segfault ***
address 0x7f804f9255c8, cause 'invalid permissions'
*** caught segfault ***
address 0x7f8046361000, cause 'invalid permissions'
I have read the other threads but I am still not sure about this. It seems nobody encountered this before. Any ideas?
Thanks in advance!