I’m new to QIIME and still learning so sorry if this is an obvious question/answer.
I’ve just run the DADA2 code and got the following outputs:
I’m trying to run the next step from the Moving Pictures tutorial (the metadata tabulate):
qiime metadata tabulate
However when I run that line of code with my stats-dada2 output this error message comes up in bold red letters:
There was an issue with loading the file stats-dada2-16Strim1.qza as metadata:
Metadata file must be encoded as UTF-8 or ASCII. The following error occurred when decoding the file:
’utf-8’ codec can’t decode byte 0xc0 in position 14: invalid start byte
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: https://docs.qiime2.org/2018.8/tutorials/metadata/
I am slightly confused as to why the error appears and what it means? Do I need to load my personal metadata file I’ve created into QIIME first? (If so why is that not a step in the tutorial?).
I’ve been through the plugins to see if there is extra code I should add but I’m not sure what I need to/if I need to.
Any help would be great , thanks, Grace