I have a lot of problems trying to import demultiplexed fastq files and I need help
I have a folder with several paired end samples, thus I create a manifest file (see below)
then I run (and I see the next error)
I tried with different types of manifest files, this mean txt, tsv…but always I obtain errors. In the same way Itried with format Phred33V2, 64V2 (also just Phred33 and Phred 64) Casava, but nothing works.
I tried to put the manifest file in the same folder than the sequences, also I tried a zip folder of the sequences, but according the type of change that I do, I can obtain:
- the manifest file is not a …Phred33 or Phreed64 or Casava…
- in addition to above, “Filepath on line 1 and column “forward-absolute-filepath” could not be found”
- sometymes I see …"expected forward-absolute-filepath, reverse-absolute-filepath… but observed expected forward-absolute-filepath, treverse-absolute-filepath
Also according some answers in the forum I tried with a manifest in which I prepare instead forwar and reverse columns, just direction, so the first line for file 1 is forward and the second is reverse, but the result is the same.
Please tell me if you have some idea how to fix this problem
Thank you for your help