Error rates could not be estimated -- unsure why

Hello Alex,

Bad news, it's an issue with dada2 and the iSeq quality scores.

The iSeq uses Illumina's new binned quality scores (official Illumina docs PDF), and I can clearly see that binning in your quality score plots.

Check out this forum thread and this GitHub issue for possible workarounds.

Good luck! Please let us know if you find a solution that works for you!