Error: QIIME 2 has no plugin/command named 'itsxpress'. Did you mean 'q2-itsxpress'?
I want to install the itsxpress in qiime2.
So I looked for tutorials and ran the following code：
source activate qiime2-2023.5
conda install -c bioconda itsxpress
pip install q2-itsxpress
qiime dev refresh-cache
qiime itsxpress
It seemed all worked.
but when start to run following code.The error appeared.
qiime itsxpress trim-pair-output-unmerged
--i-per-sample-sequences Import/Untrimmed_files/import_descriptive_mycobiome_article_sequencing_run_1.qza
--p-region ITS1
--p-taxa F
--p-cluster-id 1.0
--p-threads 4
--o-trimmed Trimmed/trimmed_descriptive_mycobiome_article_sequencing_run_1.qza
Error: QIIME 2 has no plugin/command named 'itsxpress'. Did you mean 'q2-itsxpress'?
Did I install itsxpress successfully? what is the problem?
So running qiime itsxpress worked after installation, but later when using it for an actual command it errored? Did you change conda environments between these two steps?
Which installation instructions did you follow? There are some older tutorials on this forum that differ a little from the instructions on github. If you didn't already, I would try using the those latest instructions.
Thank you for your reply.
I used this installation instruction: “ https://library.qiime2.org/plugins/q2-itsxpress/8/ ”
There is a problem I forgot to tell you
When I ran “ qiime itsxpress”
I just got “Error: QIIME 2 has no plugin/command named 'itsxpress'. Did you mean 'q2-itsxpress'?”
The following diagram shows the all code in action
“mamba install -c bioconda itsxpress” I will try it but I have no “mamba”，it gonna take a while.
Sorry，I am a beginner in this field. I have some problems.
1.What's the difference between q2-itsxpress and itsxpress？Which one do I mainly need?
2.Is the latest version of qiime2 compatible with itsxpress？
q2-itsxpress is how we refer to the QIIME 2 plugin of the tool but when you are in a QIIME 2 env you should only need to refer to itsxpres. If order to run itsxpres in QIIME 2 you need to install q2-itsxpres.
I am not sure, however there example docs use 2022.8 so that is a pretty safe bet.
I changed the code to
qiime q2-itsxpress trim-pair-output-unmerged
--i-per-sample-sequences Import/Untrimmed_files/import_descriptive_mycobiome_article_sequencing_run_1.qza
--p-region ITS1
--p-taxa F
--p-cluster-id 1.0
--p-threads 4
--o-trimmed Trimmed/trimmed_descriptive_mycobiome_article_sequencing_run_1.qza
ERROR:
Plugin error from q2-itsxpress:
cannot unpack non-iterable bool object
Debug info has been saved to /tmp/qiime2-q2cli-err-w8_lxhh9.log
I have solved this problem in my another thread dedicated to differnt bug (ITS extraction error - Missing sequence for record beginning on line 17 - #11 by salias) and I got rid of your problem by removing all older versions of ITSexpress (I suppose, maybe conflicting???) and I reinstalled ITSexpress on ver. 2.1.1. (lately 2.1.3.) in environment qiime2-amplicon-2023.9. Good luck
NOT 
(don't do this).