Hi,
I am using the PICRUSt tutorial mentioned here https://github.com/picrust/picrust2/wiki/q2-picrust2-Tutorial.
For qiime fragment-insertion sepp command I had given my aligned-rep-seq.qza file and 99 otus aligned which I have downloaded from here: ftp://greengenes.microbio.me/greengenes_release/gg_13_5/gg_13_8_otus.tar.gz. I have imported this database using the commands mentioned here https://docs.qiime2.org/2018.11/tutorials/importing/, where I have used the import command for importing aligned sequences. This is the command line I have given in PICRUSt plugin for placing the ASV:
qiime fragment-insertion sepp --i-representative-sequences aligned-rep-seqs.qza
âp-threads 20 --i-reference-alignment 99_otus.qza
âi-reference-phylogeny rooted-tree.qza \
âoutput-dir tutorial_placed_out
I am getting the following error after I ran this command:
Parameter ârepresentative_sequencesâ received an argument of type FeatureData[AlignedSequence]. An argument of subtype FeatureData[Sequence] is required.
Following is the error log report:
Traceback (most recent call last):
File â/home/gene/sankar/miniconda3/envs/qiime2-2018.11/lib/python3.5/site-packages/q2cli/commands.pyâ, line 274, in call
results = action(**arguments)
File ââ, line 2, in sepp
File â/home/gene/sankar/miniconda3/envs/qiime2-2018.11/lib/python3.5/site-packages/qiime2/sdk/action.pyâ, line 199, in bound_callable
self.signature.check_types(**user_input)
File â/home/gene/sankar/miniconda3/envs/qiime2-2018.11/lib/python3.5/site-packages/qiime2/core/type/signature.pyâ, line 301, in check_types
name, kwargs[name].type, spec.qiime_type))
TypeError: Parameter ârepresentative_sequencesâ received an argument of type FeatureData[AlignedSequence]. An argument of subtype FeatureData[Sequence] is required.
Could you please help me to resolve this issue.
Regards
Sree