Hi there! I am getting the following error when trying to import my Ion Torrent samples into QIIME2. Note they have been subjected to ITSxperss.
When I run qiime tools import --input-path /isilon/cfia-ottawa-fallowfield/users/girouxeml/gladishd/pirl_working/OW_S5-0143-161/qiime2_import_manifest.tsv --output-path /isilon/cfia-ottawa-fallowfield/users/girouxeml/gladishd/pirl_working/OW_S5-0143-161/qiime2/demix-single-end.qza --input-format SingleEndFastqManifestPhred33V2
I get error output: "There was a problem importing /isilon/cfia-ottawa-fallowfield/users/girouxeml/gladishd/pirl_working/OW_S5-0143-161/qiime2_import_manifest.tsv:
/scratch/q2-SingleLanePerSampleSingleEndFastqDirFmt-qo9hb3zd/OW-ITSF-2020-161-Plate114-208-B73_72_L001_R1_001.fastq.gz is not a(n) FastqGzFormat file:
Missing sequence for record beginning on line 9"
Please note that I am running qiime2-2021.2 in a conda environment.
I am not sure where to go. I have done many analyses like this before using similarly formatted manifest files but now it is not working for some reason. I will appreciate any help.
Thank you in advance for your time.