Hi Qiime2
Upon passing the following command
qiime cutadapt trim-paired --i-demultiplexed-sequences /Volumes/ARMS/COI/Run1_all_data/demux_qiime/R1_1/per_sample_sequences.qza --p-cores 8 --p-front-f GGWACWGGWTGAACWGTWTAYCCYCC --p-front-r TAIACYTCIGGRTGICCRAARAAYCA --o-trimmed-sequences demux_trimmed.qza
this error came up.
Plugin error from cutadapt:
Command ‘[‘cutadapt’, ‘–cores’, ‘8’, ‘–error-rate’, ‘0.1’, ‘–times’, ‘1’, ‘–overlap’, ‘3’, ‘-o’, ‘/var/folders/vs/0vv4fwcd6936b33m07w7ylwc0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-s5ozxuw3/JBI01BDMSOM1_ATCGAC_L001_R1_001.fastq.gz’, ‘-p’, ‘/var/folders/vs/0vv4fwcd6936b33m07w7ylwc0000gn/T/q2-CasavaOneEightSingleLanePerSampleDirFmt-s5ozxuw3/JBI01BDMSOM1_ATCGAC_L001_R2_001.fastq.gz’, ‘–front’, ‘GGWACWGGWTGAACWGTWTAYCCYCC’, ‘-G’, ‘TAIACYTCIGGRTGICCRAARAAYCA’, ‘/var/folders/vs/0vv4fwcd6936b33m07w7ylwc0000gn/T/qiime2-archive-tuc9lfix/281735d3-8953-4a79-b872-6b693b18eb5c/data/JBI01BDMSOM1_ATCGAC_L001_R1_001.fastq.gz’, ‘/var/folders/vs/0vv4fwcd6936b33m07w7ylwc0000gn/T/qiime2-archive-tuc9lfix/281735d3-8953-4a79-b872-6b693b18eb5c/data/JBI01BDMSOM1_ATCGAC_L001_R2_001.fastq.gz’]’ returned non-zero exit status 2
Debug info has been saved to /var/folders/vs/0vv4fwcd6936b33m07w7ylwc0000gn/T/qiime2-q2cli-err-_1jh8hhs.log
the artifact file for this command is generated through “qiime tools import --type MultiplexedPairedEndBarcodeInSequence” and “qiime cutadapt demux-paired”. Also this is a dual indexing COI samples.
Iam not really sure what happened, would be good to hear from folks if they have any ideas on this.
Best
Aji