Hello everyone. I am fairly new to DNA sequencing and Qiime but I am working on a project using Qiime2 on the EDGE bioinformatics interface. I've ran this sequence before with no issues but now I get an error saying "Barcode Fastq File: Input error. FASTQ format required." Here are my parameters:
Amplicon Type: 16s V3-V4 (SILVA)
Reads Type: De-multiplexed Reads
Metadata Mapping File: MyUploads/mapping_file_RWandPR.xlsx
Barcode Fastq File: MyUploads/manifest_file.xlsx
Quality offset: Phred+33
Quality Control Method: DADA2
Trim Forward: 230
Trim Reverse: 200
Sampling Depth: 10000
Error: Barcode Fastq File: Input error. FASTQ format required.
Any help is greatly appreciated! Thanks!