Error assigning taxonomy with UNITE

[Andrew_Richards]

Hello all,

I am trying to assign taxonomy using the UNITE database files and I am getting the following error:

There was a problem importing sh_taxonomy_qiime_ver8_99_02.02.2019.txt:

sh_taxonomy_qiime_ver8_99_02.02.2019.txt is not a(n) TSVTaxonomyFormat file

I tried using 97, 99, and developer.txt files. The FeatureData[Sequence] command works fine but not for taxonomy.

Here is my code:

Andres:thesis-data-2019 Andrew$ qiime tools import --type 'FeatureData[Taxonomy]' --input-path sh_taxonomy_qiime_ver8_99_02.02.2019.txt --output-path unite.taxonomy.qza

Should I try renaming the files?

Thanks

[Nicholas_Bokulich]

@Andrew_Richards

sh_taxonomy_qiime_ver8_99_02.02.2019.txt is not a(n) TSVTaxonomyFormat file

Must not be the correct format — most likely because it has no header so you need to use the HeaderlessTSVTaxonomy format.

See an example (and some other tricks to using the UNITE database for fungal classification) in this tutorial:

Fungal ITS analysis tutorial

Fungal ITS analysis, mock communities, and more fun NOTE: This tutorial was written in a QIIME 2 2018.11 environment. It is not guaranteed to work with earlier or later versions of QIIME 2. This tutorial was compiled as a working exercise for a QIIME 2 workshop in December 2018, and does not represent the only possible fungal ITS workflow with QIIME 2, or even a benchmarked protocol recommendation. See other tutorials, e.g., the q2-itsxpress tutorial for other fungal ITS analysis options in QI…

Good luck!