Do we still need to normalize feature table for beta-diversity?

[colinbrislawn (Colin J Brislawn)]

Good afternoon,

@junfei_guo

still need to normalize the feature table

Yes! And rarefaction is also possible in Qiime 2.

See this conversation here:

Feature Table Normalization Strategies in QIIME2

Hi Nate, Adding alternative normalization strategies has been on our radar for a while. We have an open issue here to track progress. We will post here with updates when those are added to a future QIIME2 release. However, non-rarefying-based normalization is built in to some QIIME2 analyses, e.g., ANCOM for differential abundance testing. So separate normalization may not be necessary (or even undesirable), depending on your goals. For now, rarefying is the only normalization method built in…

Colin