I am a beginner of qiime2, and please let me know your advise.
I want to analyze my Sanger sequenced data by qiime 2 pipeline.
After I imported a combined fasta file which was made by the command, “add_qiime_labels.py” under qiime1 environment, I commanded “vsearch” for dereplicating/clustering with qiime2.
Using rep-seqs.qza and table.qza, I carried out generating .a tree for phylogenic analyses(aligned-rep-seqs.qza, masked-aligned-rep-seqs.qza, unrooted-tree.qza, rooted-tree.qza).
When I proceed to the next step of diversity core metrics process, I stacked.
Would you give me some advice?
[Alpha and beta diversity analysis]
qiime diversity core-metrics-phylogenetic
Plugin error from diversity:
feature_ids must be present as tip names in
feature_ids not corresponding to tip names (n=903):
I referred similar topics about this error message(filtering?), but I can not identify the solution.
virtual box 6.1.6, QIIME 2 Core - 2020.2 on win10pro.
Miniconda 4.6.14(to prepare for qiime1 with qiime2, degraded)
Qiime 1 Forum › post split fasta file import
mapping file was validated as no error.