Hey guys, I have raw sequence which had been sequenced on Ion Torrent machine. I had followed the pipeline provided for Ion-torrent sequences and I had also done with denoise-single command with same parameters mentioned in the pipeline. When I ran the command with denoise-pyro it took longer time and when I done with denoise-single it took some time, I have two questions
A) Why the denoise-pyro took longer time.?
B) When I compared both the results there was only an small change in the frequency so can we conclude that we can either commands…? (i.e can we use denoise-single with parameter p-trim-left 15 and use the representative sequences for further analysis)
I am new to Bioinfomatics analysis especially QIIME2