I ran DADA2 on the sample regardless of the quality and from the stats, looks like many samples are filtered out and lesser reads are considered as non chimeric. Is this right?
dada2-stats.qzv (1.2 MB)
We are still thinking if we should proceed with only forward reads. 
But may I know what is the significance/implication(?) of this? We know that the region (V3-V4) is around 460 bp but with only forward read, it will consider only half of it: 230 bp?
Appreciate if you can share me your thought on this. 