I am experiencing an error with a new dataset I just received and I don’t understand how to fix it. I can import and run the demultiplexing steps, but when I try to summarize the demultiplexing I get an error.
Here are the commands I have run:
qiime tools import --type EMPSingleEndSequences --input-path /Users/Molly-Mac/My_FILES/16s_july26_Run/Data --output-path SmPro_PlasmidTrial_sequences.qza
qiime demux emp-single --i-seqs /Users/Molly-Mac/My_FILES/6s_july26_Run/SmPro_PlasmidTrial_sequences.qza --m-barcodes-file /Users/Molly-Mac/My_FILES/16s_july26_Run/SmPro_Mapping.txt --m-barcodes-column BarcodeSequence --o-per-sample-sequences Demulti_SmPro_PlasmidTrial_sequences3.qza
qiime demux summarize --i-data /Users/Molly-Mac/My_FILES/16s_july26_Run/Demulti_SmPro_PlasmidTrial_sequences3.qza --o-visualization Dmultivis_SmPr_PlasmidTrial3.qzv
Plugin error from demux:
join() argument must be str or bytes, not ‘float’
Debug info has been saved to /var/folders/kk/ppfc6f0x2v36xxkd7lpln88w0000gn/T/qiime2-q2cli-err-qu75omdf.log
Is there a standard reason for this error to occur? I can seem to figure out how to file where the debug info has been saved so if there is any guidance on that I’d be happy to provide it.