Demultiplexing Help

[thermokarst]

Hey @Dean! We have seen a few reports like this in the last couple of days, for example:

Cutadapt thinks my fastq.gz files aren't fastq.gz

I am very new to using Qiime2, and I don't have any coding experience. I have single end, already demultiplexed reads produced by an Ion Torrent PGM. I have been able to import these reads using the fastq manifest format, and everything went fine. I was also able to import them as Casava format. The below error happens with both formats. I have tried to trim my primers using this command: qiime cutadapt trim-single --i-demultiplexed-sequences MC1_run1_demux.qza --p-adapter CTTCCGGTACACTTACCAT…

This thread can fill you in on the details. We are working on the issue right now and will keep you posted. Thanks!