Hi everyone! I never used Qiime2 before and I was trying to understand how I can demultiplex my reads. I have two fasq.gz file of paired-end reads from Illumina Miseq 2x150 sequencing. My reads have a sample 8 bases sample tag and 2/4 leading Ns. Both forward and reverse primers are tagged, so I u…

For importing, check out this portion of our importing tutorial . From the information that you have provided, that looks like the correct approach. Since it sounds like you are already an experienced researcher, I think you will find this page very helpful for understanding the QIIME 2 workflow and…

Demultiplexing dual-barcoded paired-end sequences from freshwater metabarcoding Illumina sequencing