Hi, I'm trying to demultiplex my joined reads sequences. The information about my sequences is as follow: the raw data files are –full.fasta & -full.qual, which contain the raw sequence data information and have primers and barcodes. (Since I didn't get any other sequence file, I guess theses seq…

*this is a supplement for this question: actually, I also have two files: –pr.fasta, -pr.qual, which are Sequencing information without the primer-barcode used in our analysis. The sample names from which each sequence is derived is encoded in the definition line of each sequence and short files < …

Hey there @nan_wang ! [image] nan_wang: actually, I also have two files: –pr.fasta, -pr.qual, which are Sequencing information without the primer-barcode used in our analysis. The sample names from which each sequence is derived is encoded in the definition line of each sequence and short files <…

Soryy, I didn't explain my data clearly. Actually, my data was PGM sequencing data. I've merged the barcode&primer-removed sequence data (fasta) and qualitydata (.qual) into .fastq data like this: [35] I've read the post from How to do multiplex of Ion torrent sequences . I guess this solution cou…

[image] nan_wang: now all my samples are in one fastq fil Yep, this is what I was referring to above --- QIIME 2 doesn't have a way for you to demux samples without barcodes in the reads (or, in the case of EMP data, a separate barcodes file). [image] nan_wang: Also, I’ve merged the barc…

Thanks a lot for your quick reply! Actually, I successfully imported data just now. but I have one more question Demultiplex joined reads sequences - #11 . Should I worry about that? Thank you very much!

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Demultiplex joined reads sequences

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demux, paired-end

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