Hi @jwdebelius,
Here is the path file for the ERR264394 which are the reads before they were split into forward (_1) and reverse (_2): 
This is the file path I used in the code I sent this morning which is the split reads 
Should I be calling upon the non-split reads (image 1) to pull the files into qiime? That is what I was doing previously but it did not work.
Thanks for the help!
Christina