data import type and directory format

Hi @cal1037,

Could you please show the print out of

ls /mnt/home/ernakovish/ca1037/devries_files/ | grep ERR264394

Id like to check. the path to that file. …And If Ive mistyped because Im trying to copy off your images, please use the correct address that’s the first part of the manifest path.

Best,
Justine

Hi @jwdebelius,

Here is the path file for the ERR264394 which are the reads before they were split into forward (_1) and reverse (_2):

This is the file path I used in the code I sent this morning which is the split reads

Should I be calling upon the non-split reads (image 1) to pull the files into qiime? That is what I was doing previously but it did not work.

Thanks for the help!
Christina

Hi @cal1037,

I think you need a “/” between in the path in the manifest where you have a space.

So, for line 1, the forward path should be
/mnt/home/ernakovish/ca1037/devries_files/import_devries_split/ERR264394_1.fastq.

Best,
Justine

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