Hi, I am new to working with micorbial data and have been searching through the qiime forum for answers but I haven’t found anything that fits my exact problem.
I have been working to import data process it on Qiime2 2020.2. The data is from a paper that uploaded sequences to NCBI SRA. I downloaded 22 files (of 239) which were part of one run from NCBI into a directory. The general file format is EERfileID.fastq.gz format. I have them on my directory but I cannont import them to qiime2 without errors.
This is the code I have run:
qiime tools import
but I keep getting this error:
The main thing comfusing me is the “Semantic type PairedEndSequences does not have a compatible directory format” error. I don’t know if the data I have is in the correct format/the format it should be in. Based on the import, atacma, and moving pictures tutorial fastq.gz formats are alright to use/import to qiime. Is there antoher format the files should be in?
The data should be paired end reads but I cannot tell R1 from R2 and don’t know if I need to specify this or if doing so would remove the error. If I need to import all 239 files from NCBI I can but am not sure if that will fix the directory format.
If anyone has advice regarding this/importing from NCBI, I’d apprecaite the help!