Thanks for answering the question. so can I run the removal of primers with quality control together or I have to run them seperately? When I run the denoise-paired in quality control, if I use the following commands, is it correct?
qiime dada2 denoise-paired --i-demultiplexed-seqs demux-paired-end.qza --p-trim-left-f 17 --p-trunc-len-f 250 --p-trim-left-r 21 --p-trunc-len-r 250 --o-table bing-dada2-table.qza --o-representative-sequences bing-data2-rep-seqs.qza --verbose
Bing