dada2 trim and trunq means

[medhanlee0721 (Han Lee)]

Hi guys i want to know if i understand correct meaning.
my sequencing was single-end sequence with V3-V4 region
I want to trim and trunc dada2 file

Please help if this is correct code matched with meaning

1. p-trim-left 296 --p-trunc-len 0
   -> eliminate nts under 295 and remain nts over 296 - 460

2. p-trim-left 0 --p-trunc-len 296
   -> eliminate nts over 297 and remain nts over 0 - 296

is this right cod that i mean to?

[lizgehret]

Hi @medhanlee0721,

I am going to close out this thread since it is a duplicate from your post here. Please see our code of conduct regarding cross-posting. We are happy to continue providing guidance but please make sure to keep each issue/question to a single post; this helps us keep things organized. Thanks!