Hello everyone! I used dada2 to denoise my paired data. However, when I checked the file dada2_stats.qzv, I found that some samples did not filter. I want to know where went wrong.
I used these code and outputted three files without an error.
time qiime dada2 denoise-paired --i-demultiplexed-seqs paired-end-demux.qza --p-n-threads 15 --p-trunc-len-f 298 --p-trunc-len-r 286 --o-table dada2_table.qza --o-representative-sequences dada2_rep_set.qza --o-denoising-stats dada2_stats.qza
Then I used:
qiime metadata tabulate
Finally, I got the file like the picture.
I tried the denoising step again, and got the same result.
Can anyone help me with this problem?