DADA2:low non-chimeric

Dear all:
When I use DADA2 to process my data with qiime2-2021.4 , I got very low non-chimerism. My sequencing platform is Novaseq. This is demux picture.


The command I use istime qiime dada2 denoise-paired \ --i-demultiplexed-seqs demux-paired-end.qza \ --p-trim-left-f 0 --p-trim-left-r 0 \ --p-trunc-len-f 0 --p-trunc-len-r 0 \ --o-table table.qza \ --o-representative-sequences rep-seqs.qza \ --o-denoising-stats denoising-stats.qza
I got very low percentage non-chinmeric.1630381582(1)
Why does this happen? Is there any way I can improve it?
Thank you!
Sincerely

Hi @Susun
Did you already check this post with a similar issue?
There are also some links to similar topics, maybe you'll find it useful.

1 Like

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.