Can anyone advise me about this issue? after running the command below
qiime dada2 denoise-paired
–p-trim-left-f bp of primer F
–p-trim-left-r bp of primer R
–p-trunc-len-f length of best quality
–p-trunc-len-r length of best quality
–p-n-reads-learn How many times it tries
–p-n-threads unlimited (0)
I got only few merged sequences. would be possible to adjust the command to get more sequences? Ex the input was 29004 but only 8 sequences were merged.
See the attached file.