dada2 failed while running nextseq data

Hello Rachel,

Welcome to the forums! :qiime2:

Thank you for including ALL the details in your post. This is very helpful!

I think these two things are related: binned quality scores :point_down:


and

Error rates could not be estimated

Take a look at this thread from the DADA2 developer: NovaSeq and Dada2 incompatibility. - #12 by benjjneb

Also check out the alternative methods outlined here: NovaSeq data in QIIME2

Keep us posted on your progress and what you try next!

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