I have similar problem at Dada2 step (“Duplicate sample IDs!”) of QIIME2 pipeline. My manifest file has no duplicate entries?
Start - Mon May 1 09:26:08 EDT 2017
2 R version 3.3.1 (2016-06-21)
3 Loading required package: Rcpp
4 Warning messages:
5 1: multiple methods tables found for ‘arbind’
6 2: multiple methods tables found for ‘acbind’
7 3: replacing previous import ‘IRanges::arbind’ by ‘SummarizedExperiment::arbind’ when loading ‘GenomicAlignments’
8 4: replacing previous import ‘IRanges::acbind’ by ‘SummarizedExperiment::acbind’ when loading ‘GenomicAlignments’
9 5: multiple methods tables found for ‘left’
10 6: multiple methods tables found for ‘right’
11 DADA2 R package version: 1.1.7
12 1) Filtering ...................................................................................................................................................... ................................................................................................................................................................... ................................................................................................................................................................... ................................................................................................................................................................... ........................................................
13 2) Learning Error Rates
14 2a) Forward Reads
15 Initial error matrix unspecified. Error rates will be initialized to the maximum possible estimate from this data.
16 Initializing error rates to maximum possible estimate.
17 Sample 1 - 39375 reads in 7338 unique sequences.
18 Sample 2 - 67127 reads in 11819 unique sequences.
19 Sample 3 - 49275 reads in 17656 unique sequences.
20 Sample 4 - 111841 reads in 18908 unique sequences.
21 Sample 5 - 66061 reads in 13826 unique sequences.
22 Sample 6 - 97741 reads in 20705 unique sequences.
23 Sample 7 - 86889 reads in 14801 unique sequences.
24 Sample 8 - 94258 reads in 17576 unique sequences.
25 Sample 9 - 84723 reads in 19152 unique sequences.
26 Sample 10 - 78928 reads in 11940 unique sequences.
27 Sample 11 - 94925 reads in 19716 unique sequences.
28 Sample 12 - 247547 reads in 53555 unique sequences.
29 selfConsist step 2
30 selfConsist step 3
31 selfConsist step 4
32 selfConsist step 5
33
34
35 Convergence after 5 rounds.
36 2b) Reverse Reads
37 Initial error matrix unspecified. Error rates will be initialized to the maximum possible estimate from this data.
38 Initializing error rates to maximum possible estimate.
39 Sample 1 - 39375 reads in 11958 unique sequences.
40 Sample 2 - 67127 reads in 22933 unique sequences.
41 Sample 3 - 49275 reads in 17223 unique sequences.
42 Sample 4 - 111841 reads in 33476 unique sequences.
43 Sample 5 - 66061 reads in 19661 unique sequences.
Sample 6 - 97741 reads in 36454 unique sequences.
45 Sample 7 - 86889 reads in 24370 unique sequences.
46 Sample 8 - 94258 reads in 33442 unique sequences.
47 Sample 9 - 84723 reads in 32011 unique sequences.
48 Sample 10 - 78928 reads in 21581 unique sequences.
49 Sample 11 - 94925 reads in 34709 unique sequences.
50 Sample 12 - 247547 reads in 76285 unique sequences.
51 selfConsist step 2
52 selfConsist step 3
53 selfConsist step 4
54 selfConsist step 5
55
56
57 Convergence after 5 rounds.
58
59 3) Denoise remaining samples ...................................................................................................................................... ................................................................................................................................................................... ................................................................................................................................................................... ................................................................................................................................................................... ............................................................
60 The sequences being tabled vary in length.
61 4) Remove chimeras (method = pooled)
62 5) Write output
63 Traceback (most recent call last):
64 File "/home/adinasarapu/anaconda2/envs/qiime2-2017.4/lib/python3.5/site-packages/q2cli/commands.py", line 218, in __call__
65 results = action(**arguments)
66 File "<decorator-gen-241>", line 2, in denoise_paired
67 File "/home/adinasarapu/anaconda2/envs/qiime2-2017.4/lib/python3.5/site-packages/qiime2/sdk/action.py", line 171, in callable_wrapper
68 output_types, provenance)
69 File "/home/adinasarapu/anaconda2/envs/qiime2-2017.4/lib/python3.5/site-packages/qiime2/sdk/action.py", line 248, in _callable_executor_
70 output_views = callable(**view_args)
71 File "/home/adinasarapu/anaconda2/envs/qiime2-2017.4/lib/python3.5/site-packages/q2_dada2/_denoise.py", line 174, in denoise_paired
72 return _denoise_helper(biom_fp, hashed_feature_ids)
73 File "/home/adinasarapu/anaconda2/envs/qiime2-2017.4/lib/python3.5/site-packages/q2_dada2/_denoise.py", line 77, in _denoise_helper
74 table.update_ids(sid_map, axis='sample', inplace=True)
75 File "/home/adinasarapu/anaconda2/envs/qiime2-2017.4/lib/python3.5/site-packages/biom_format-2.1.5-py3.5-linux-x86_64.egg/biom/table.py", line 1069, in update_id s
76 errcheck(result)
77 File "/home/adinasarapu/anaconda2/envs/qiime2-2017.4/lib/python3.5/site-packages/biom_format-2.1.5-py3.5-linux-x86_64.egg/biom/err.py", line 472, in errcheck
78 raise ret
79 biom.exception.TableException: Duplicate sample IDs!
80
81 Plugin error from dada2:
82
83 Duplicate sample IDs!
84
85 See above for debug info.
86 Running external command line application(s). This may print messages to stdout and/or stderr.
87 The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.
88
89 Command: run_dada_paired.R /tmp/tmp.YaC65WXhrx/tmpaz1m468_/forward /tmp/tmp.YaC65WXhrx/tmpaz1m468_/reverse /tmp/tmp.YaC65WXhrx/tmpaz1m468_/output.tsv.biom /tmp/tmp .YaC65WXhrx/tmpaz1m468_/filt_f /tmp/tmp.YaC65WXhrx/tmpaz1m468_/filt_r 200 200 20 20 2.0 2 pooled 1.0 60 1000000