DADA2 denoise-single exit code 1

Hello Im going through the qiime2 tutorial files and getting the same message:

qiime2-moving-pictures-tutorial zc$ qiime dada2 denoise-single   --i-demultiplexed-seqs demux.qza   --p-trim-left 0   --p-trunc-len 120   --o-representative-sequences rep-seqs-dada2.qza   --o-table table-dada2.qza

Plugin error from dada2:

  Command '['run_dada_single.R',
  diu7c2ek/bfcda1b6-ab77-4465-99a8-ae24680f6b27/data', '/var/folders/yk/
  '/var/folders/yk/mbw69r451vq7ylyp2d96jwlw0000gn/T/tmp222mwism', '120',
  '0', '2.0', '2', 'consensus', '1.0', '1', '1000000']' returned non-
  zero exit status 1

Debug info has been saved to /var/folders/yk/mbw69r451vq7ylyp2d96jwlw0000gn/T/qiime2-q2cli-err-oz6qc3zy.log.

Hi @ZC_C, in order for us to assist you, can you please provide the following:

  1. The version of QIIME 2 you are running.
  2. The detailed error log (you could attach the file listed above at /var/folders/yk/mbw69r451vq7ylyp2d96jwlw0000gn/T/qiime2-q2cli-err-oz6qc3zy.log, or re-run with --verbose and copy-and-paste the output here).


Hi thermokarst thanks for your reply, I have noticed that my R dada2 was missing and after reinstalling R and upgrading to the Qiime 2 to 2017.7 it started working.

1 Like

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.