DADA2- an error accrued

[shanif3 (Shani Finkelstein)]

Hi!
I got an error during DADA2, its a paired-end data.
Attached:

Thanks in advance!

[cherman2 (Chloe Herman)]

Hi @shanif3,
Take a look at this thread and see if the advice can help!

DADA2: Mismatched forward and reverse reads

Thank you for the reply! It looks like these were the rawest form of the reads we received, the sequencing core they came from did demultiplex them before they were sent our way. So the fact they were already demultiplexed might be why I am running into this error. Thank you for the help and I will keep working at trying to find the root of the problem and if not can run it through Deblur instead.