In an effort to cut down on email notifications, shifting any community discussion from CZI-CABANA Microbiome Bioinformatics with QIIME 2 Workshop (our first-ever online QIIME 2 workshop!) to this thread. Ask away!

Same for me, the YouTube link is showing as restricted today when yesterday I was able to view it just fine.

Maybe you try searching "QIIME 2" on YouTube

The video is now working but the audio is shut down, can you guys listen to it? Thanks for this chance!

yes i can listen to it, very smoothly

Hello @chiara ! I am currently listening to the youtube stream and there is audio. I would suggest checking your personal set up. Hope this helps!

To add to the discussion about raw sequences and what is inside; I think you can check the length of the sequences to see if it is longer than the expected size. Let's say read length is 2x250. Then, if we have sequences longer than 250 bp, doesn't it mean that there are barcodes or index or someth…

Hi @the_dummy , I agree on fastqc. To answer your question, not necessarily. It may depend on the prep kit and where are sequencing adapters. In my experience, the length of 250 bp includes everything, sequencing adapters (depending on kit ), barcodes as well as PCR primers and low-quality tails. He…

Hi @llenzi , In that case, I'm mistaken to rely only on my experiences. Thank you very much!

Hi, Warm greetings! Thank you for your response and suggestion. I will keep that in mind next time I post a question. Am curiously waiting for some answers too. That was very helpful. Thank you very much. My sincere regards.

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