Hi,
It will be highly appreciated if it is possible to share the recording of yesterday’s session before today’s session since I had missed out the metadata segment of the session due to the difference in our time zones.
I am requesting this since I am a beginner in this area and it will be very difficult for me to follow the next segment.
I had tried for registration but seats were unavailable and hence I had opted for the free-viewing option.
If you start the livestream and then pause it - it can be paused for any length of time and you can come back and watch it (ie the next day if time zones are an issue). I tested this last night and even with the stream hiccups the ‘unpaused live’ stream was flawless. If you do not start it when the stream begins, I do not think it is possible to go back and access it. Hope this helps
To add to the discussion about raw sequences and what is inside;
I think you can check the length of the sequences to see if it is longer than the expected size. Let’s say read length is 2x250. Then, if we have sequences longer than 250 bp, doesn’t it mean that there are barcodes or index or something else?
Also, an option out of Q2, FastQC should show if there are Illumina barcodes in fastq files if I’m remembering correctly.
Lastly, yes, there is not a specific way to tell what is inside our files if it is not delivered with right info.
I agree on fastqc. To answer your question, not necessarily. It may depend on the prep kit and where are sequencing adapters. In my experience, the length of 250 bp includes everything, sequencing adapters (depending on kit ), barcodes as well as PCR primers and low-quality tails. Hence, for me when I saw 250 bp it means these are as raw as possible (for a 2x250 bp MiSeq run at least)
Cheers
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jwdebelius
(Justine Debelius)
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