Hello,

I am working on a custom OTU clustering algorithm and trying to measure sensitivity, specificity.

Should I calcualte the True Positive (TP), False Positive (FP), False Negative(FN) and True Negative values for each OTU and get the average, or it should calculate it for all. If so, then

How can I calculate True Negatives? Since it is different for each OTU cluster?

My evaluation is :

If a sequence is correctly clustered then it is TP

If a sequence is not correctly clustered then it is FP

If a sequence could not be clustered and left alone it is FN

now, for True Negatives I can calculate them for each OTU :

TN= N-(TP+FP+FN)

Should I simply calculate sensitivity and specifity for each OTU alone and then take an average ?

I really appreciate your help for me to understand this concept.

I hope someone can help me.

Thank your