Constructed from biom file

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1

Hi, I am at the last step for microbiota analysis but when I want create OTU table and write the following input :biom convert -i feature-table.biom -o feature-table.tsv --to-tsv
$ biom head -i feature-table.tsv

Schermata 2025-06-03 alle 22.27.02
Schermata 2025-06-03 alle 22.27.021142×198 11.6 KB

I obtained this results:
Constructed from biom file

#OTU ID A.C.15 A.C.2 A.C.23 A.C.25 A.C.34

9b5a325cd936b5e4f78b1e619ee1563b 12.0 0.0 0.0 581.0 27.0

05c9bbf84124fda5997f3226195fe55e 23.0 0.0 0.0 339.0 41.0

5f25f7579e119e74f17195fd46454998 0.0 0.0 1593.0 808.0 43.0

16deb77996c4d37ca051a9d5c6e4cdb3 0.0 0.0 0.0 220.0 19.0

f60caf075abaf24f04c0ac6b897f6814 0.0 0.0 0.0 0.0 0.0
Now, what I have to do?

2

Hi @DECARO

The output you are seeing looks okay to me. A feature table where rows are OTU IDs (or ASVs if you are using DADA2) and columns are your sample IDs. Values are feature counts per sample.

Well, it depends. What is your research objective? You may want to add taxonomy, measure diversity, or do any of the multiple things you can do with a feature table. Let us know what you want to do so we can help from there!

Best,

Sergio