In the code below:
time qiime dada2 denoise-paired
–i-demultiplexed-seqs trimmed-seqs.qza
–p-trim-left-f 35
–p-trunc-len-f 220 \
I want to know – does p-trunc-len-f 220 mean to cut to the position 220(from 35 to 220), or does it mean to cut from 35 to 255 in a 220 length area?