In the demultiplexed section, when I view demux.qzv I get two quality plots one for forward reads and other for reverse reads. Now I donot whether I am wrong or right. Further I donot know which parameters I used for sequence quality control?
Confused about demux.qzv for getting two quality plots i.e. for forward reads and reverse reads in the interactive quality plot tabs?
Hi @Aqleem12, thank you for reaching out. There isn’t really a right or wrong, but some downstream methods may have different expectations. In the case of
DADA2, as I understand it, quality filtering is not necessary. For
Deblur, we advise at least minimal filtering with
q2-quality-filter (e.g., the default parameters).
If you haven’t read it yet, you may be interested in Bokulich et al 2013 which explores the impact of PHRED filtering, and which forms the basis for the parameters used by
Locking this duplicate topic and moving further support here: