Confused about demux.qzv for getting two quality plots i.e. for forward reads and reverse reads in the interactive quality plot tabs?

[jairideout (Jai Ram Rideout)]

Locking this duplicate topic and moving further support here:

I donot know how to truncate paired sequence bases. How do we know about the quality of the bases is high or low?

In the demux.qzv quality plots, i.e . two quality plots one for forward and one for reverse. I donot know how to truncated both quality plots. I have attached the file. Please help me in sequence quality control I used the command as follows;qiime dada2 denoise-single --i-demultiplexed-seqs demux.qza --p-trim-left 0 --p-trunc-len 120 --o-representative-sequences rep-seqs-dada2.qza --o-table table-dada2.qza [image]