I have been able to follow the “Moving Pictures” tutorial (which is a phenomenal resource!) to perform some really interesting data analysis so far, but I want to see if I can make any additional useful comparisons. Since I’m working with the microbiome of salamanders, I want to compare my data to cutaneous bacteria of amphibians which comes in the form of a .fna file from the following link:
I have performed everything in the “Moving Pictures” tutorial on my personal data, so I have a ton of different file types to work with (e.g. rep-seqs, de novo clustered rep-seqs, tables, filtered-tables, and more). I was wondering if there was a way with qiime2 to look at the similarity between the two sets of data. So far, I’ve only imported the .fna of the common amphibian cutaneous bacteria as a FeatureData[Sequence] artifact and a FeatureTable[Frequency] artifact but have not done many different transformations to it (because I don’t know if there’s a way for me to compare them yet).
My question lies in if there is an existing way to directly take two different sets and compare, or if there’s a way to integrate them into one mega dataset and then run internal analyses based on metadata columns. In the event there’s not a way to do so in qiime2, could anyone point me somewhere where I’d be able to do such analysis?
Thanks so much, and have a wonderful day!