Combining high- and low-biomass samples in a run.

Dear all,

I was wondering whether I can have a sequencing run that contains both high and low biomass samples.

Do you think that this will lead to contaminations that can not be corrected bioinformatically or is it acceptable to have a run that contains samples of different biomass? Did you ever tested this?

Thank you,

Hi @dc718,

You might find this paper of interest:

My conclusion has always been that it's not great practice, but as always, there are under discussed layers to the contamination we get.