Clustring Autistic 16S samples

Hi everyone.

Coming from a purely biomedical background, I now have a master's project investigating clusters within autistic children's microbiome.

I am starting with ca 30 fastq samples of all autistic children and would like to investigate if there would be subgroups within these samples. What would you recommend for pipeline to do this

TIA

Hello Ahmed,

We have a long list of tutorials that show what you can do with Qiime2. Because everyone's data set is different, you will have to mix and match between these to find what fits your data.

The PD-mouse tutorial discusses several different ways to investigate groups of samples. Check it out:
https://docs.qiime2.org/2021.8/tutorials/pd-mice/#machine-learning-classifiers-for-predicting-sample-characteristics

Colin

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