Classifier of V1-V2 (7F, 338R) region with gg_99

[110147]

hello , I also want to ask some question about the classifier ,I have a batch data from the V1-2 target ,but I use the data from 515F/806R is wrong ,

so I want to train myself database ,but I meet some question.

I don't know how to slove the question .

the last I use this to do taxa for my data
qiime feature-classifier classify-consensus-blast
--i-query rep-seqs.qza
--i-reference-reads 99_otus.qza
--i-reference-taxonomy ref-taxonomy.qza
--p-maxaccepts 2
--o-classification taxonomy-blast-greengenes13-8.qza
the 99_otus.qzas from the gg and the ref-taxonomy.qza from the gg .but I find the taxa need very long and long time ,So, I hope you can tell me how to train my data about myself primers .thanks you very much ,I need youe help .

[ebolyen (Evan Bolyen)]

Hi @110147!

I believe the issue is with your reverse primer. It lists: CYIACT…
The letter “I” isn’t an IUPAC code. I think it might have just been a typo, but if not, what DNA bases is it meant to represent in your primer?