classifier does not support confidence values in rescript

[TurboQiimer]

Hi to all Qiime2 Community,

I am in the last step of using rescript. I walked through the following protocol:

Using RESCRIPt to compile sequence databases and taxonomy classifiers from NCBI Genbank

This tutorial will describe how to create custom reference databases from NCBI Genbank using RESCRIPt. Read more about RESCRIPt here: https://forum.qiime2.org/t/processing-filtering-and-evaluating-reference-sequence-data-with-rescript/15494/7 If using NCBI Genbank data, please be aware of the NCBI disclaimer and copyright notice. Citation: If you use RESCRIPt or any RESCRIPt-processed data in your research, please cite the following pre-print: Michael S Robeson II, Devon R O'Rourke, Benjami…

** I got this error in the fourth step:**

qiime rescript evaluate-fit-classifier \

**--i-sequences ncbi-refseqs.qza** \
--i-taxonomy ncbi-refseqs-taxonomy.qza \
--o-classifier ncbi-refseqs-classifier.qza \
--o-evaluation ncbi-refseqs-classifier-evaluation.qzv \
--o-observed-taxonomy ncbi-refseqs-predicted-taxonomy.qza

Plugin error from rescript:

this classifier does not support confidence values

Debug info has been saved to /tmp/qiime2-q2cli-err-84q7mc16.log

I used 2020.08 Qiime2 version. And I used filtered sequences for --i-sequences parameter.

1. Would you please tell me what exactly the problem is? Or How to solve it?
2. Can I classify the filtered taxonomy and filtered sequence with my experimental sequences using e.g. [VSEARCH-based consensus taxonomy classifier]?

Thanks
Qiimer

[SoilRotifer (Mike Robeson)]

Hi @TurboQiimer,

Can you paste all of the actual commands you ran? I just want to make sure there was no mistakes when implementing the pipeline.

Also, can you make a qzv of ncbi-refseqs-taxonomy.qza and share that? Hopefully, it won't be a large file. If it is, you can link them to me (e.g. dropbox) via a DM.