Chimeras - dada2 settings, and optimal PCR polymerase?

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We are getting somewhat high % of chimeras in our fecal microbiota data.

  • How can we modify the dada2 chimera removal parameters AND ESPECIALLY: how to evaluate what is “right”? I presume it’s possible to set it, like, too relaxed?
  • I presume also the PCR polymerase properties might matter. What are nowadays the best polymerases in this sense?
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Welcome to the forums, Mikael

Can you edit your post for me?

Specifically:
Remove all that default text, as that's intended for you to read.
Add these two sections:

  • Version of QIIME 2 you are running, and how it is installed (e.g. Virtualbox, conda, etc.)
  • What is the exact command or commands you ran? Copy and paste please.

Help us help you :handshake:

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