Since the amount of data sequenced from several samples is relatively small, there are only a few thousand sequences. I used the same DNA , the same primers and the same parameters for the second sequencing of these samples, only a few thousand sequences were obtained.
Can I combine these thousands of sequences with the sequence obtained from the first sequencing for analysis? Is this feasible? What methods can be used?
I think it is necessary to use DADA2 to denoise separately, how should the results obtained in this way be combined?